GB 5009.208-2016 Translated English of Chinese Standard. GB5009.208-2016: National Food Safety Standard - Determination of Biogenic Amine in Foodhttps://www.chinesestandard.net, 14. sep. 2019 - 17 sider This Standard specifies the determination method for tryptamine, -phenylethylamine, putrescine, cadaverine, histamine, octopamine, tyramine, spermidine and spermine content in food. This Standard is applicable to the determination of alcohol (wine, beer and yellow rice wine, etc.), condiments (vinegar and soy sauce), aquatic products (fish and its products; shrimp and its products), biogenic amine in meat. |
Indhold
| 3 | |
Liquid Chromatography | 4 |
Instruments and Equipment | 8 |
Expression of Analytical Result | 11 |
Spectrophotometry | 13 |
Analytical Procedures | 14 |
Result Calculation | 15 |
Precision | 16 |
Appendix A Liquid Chromatogram of 9 Biogenic Amine Standard Solutions and Internal Standard Derivatives | 17 |
Almindelige termer og sætninger
0.01 mol/L ammonium 0.1 mol/L hydrochloric 100 mL volumetric 40 C water absorb 1.0 mL accurately weigh-take acetic acid acetone acid to dilute Add 1 mL ammonium acetate solution arithmetic mean azo reagent biogenic amine standard cadaverine chromatographically pure conduct oscillation extraction conduct vortex oscillation constant volume Dansyl chloride derivatization Determination of Biogenic expressed in mL g of sodium hydrochloric acid solution mL of hydrochloric mL volumetric flask Mobile phase mol/L ammonium acetate mol/L hydrochloric acid n-hexane N-pentanol extract nitrogen to blow octopamine Prepare it right products and meat proper amount putrescine reach a constant right before usage sample solution shelf sodium chloride sodium hydroxide sodium hydroxide solution sodium nitrite solution 100 mg/L solution that contains soy sauce spectrophotometer spermidine spermine standard solution standard stock solutions standard substance purity standard working solution transfer trichloroacetic acid solution Trichloromethane tryptamine tyramine various biogenic amine water bath water to completely weigh-take 25 g