The Isolated Hepatocyte: Use in Toxicology and Xenobiotic Biotransformations

7-hydroxycoumarin acetaminophen acid Acosta activity adriamycin adult rat hepatocytes animal assay Biochem Biol Biophys Bucher cadmium calcium carcinogens cellular Chem chemical concentration conjugation cultured hepatocytes cytochrome P-450 cytotoxicity cytotoxins decrease DNA repair DNA synthesis drug effects enzyme epidermal growth factor fraction function genotoxic glucagon glucose glucuronidation glucuronosyltransferase glutathione hepatic hepatocyte cultures hepatocyte DNA hepatotoxicity hormones human hepatocytes incubated indicators induced inhibition insulin interactions intracellular investigated isolated hepatocytes isolated rat hepatocytes Kupffer cells levels lipid peroxidation liver liver cells liver lobule measured Mechanism medium metabolism metabolites Michalopoulos microsomal mitochondrial monolayer cultures mouse normal parenchymal cells partial hepatectomy perfused perfused livers pericentral pericentral areas pericentral regions periportal and pericentral Pharmacol postnatal primary cultures protein rat liver rates of glucuronidation receptors sensitive serum specific Stacey and Klaassen stimulation studies substrate suspensions tion tissue toxicity Toxicol trypan blue UDPGA uptake urea viability vitro vivo Williams xenobiotics