Protein Analysis and Purification: Benchtop TechniquesSpringer, 1996 - 434 sider This comprehensive, practical and user-friendly manual is designed to be a practical progression of experimental protocols that an inexperienced investigator may follow when embarking on a biochemical or biotechnology project. |
Indhold
An Overview of this Manual | 2 |
Tertiary Structure | 12 |
Surface Labeling | 25 |
Copyright | |
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acetic acrylamide activity affinity agarose amino acid ammonium analysis analyze antibody antigen Appendix assay band beads binding Biochem Biol Chem blot cells centrifuge chromatography cleavage column complex containing cross-linking detection detergent digestion dilute EDTA electrophoresis enzyme equilibration extract Figure final concentration fractions fusion protein gene glycoproteins gradient groups HPLC hydrophobic immobilized immunoprecipitation incubate inhibitors interactions ion exchange kinase labeling lectin ligand lysate Materials matrix membrane proteins methanol method mg/ml microfuge molecular weight molecules NaCl oligosaccharides pellet peptide phosphate phosphorylation plate PMSF polypeptide precipitate prepared protease protein of interest PROTOCOL purification PVDF radioactive reaction reagent recombinant remove residues Resuspend room temperature salt SDS sample buffer SDS-PAGE separating gel Sepharose sequence serine sodium soluble solvent specific stacking gel staining step structure sulfate supernatant target protein technique transfection transfer Tris-HCl Triton trypsin tube volume wash buffer Western blotting µg/ml