The Isolated hepatocyte: Use in Toxicology and Xenobiotic BiotransformationsE.J. Rauckman Elsevier, 2. dec. 2012 - 304 sider The Isolated Hepatocyte: Use in Toxicology and Xenobiotic Biotransformations covers the link between research on the isolated hepatocyte and the disciplines of cell culture, toxicology, metabolism, and molecular biology. This book is composed of 11 chapters and begins with an overview of the regulation of liver growth, sulfation, glucuronidation of xenobiotics in specific liver sites. The next chapters deal with toxicology studies in cultured hepatocytes from various species and the in vitro control of hepatocyte proliferation. These topics are followed by discussions of choices and methods of cytotoxicity measures; hepatoxicity of metals; the metabolism and toxicity of xenobiotics in a primary culture; and the mechanism of carcinogen-induced pleiotropic drug resistance in hepatocytes. The concluding chapters describe the in vivo and in vitro measurement of chemically-induced DNA repair in hepatocytes, as well as the genotoxicity studies with human hepatocytes. This book is intended primarily to toxicologists and researchers. |
Indhold
| 1 | |
| 21 | |
Chapter 3 Toxicology Studies in Cultured Hepatocytes from Various Species | 51 |
Chapter 4 Cytochrome P450Dependent Monooxygenase Systems in Mouse Hepatocytes | 69 |
Chapter 5 Control of Hepatocyte Proliferation in Vitro | 93 |
Choices and Methods | 119 |
Chapter 7 Metals Hepatocytes and Toxicology | 159 |
Chapter 8 The Metabolism and Toxicity of Xenobiotics in a Primary Culture System of Postnatal Rat Hepatocytes | 189 |
Chapter 9 The Analysis of CarcinogenInduced Pleiotropic Drug Resistance in Rat Hepatocytes | 215 |
Chapter 10 Measurement of Chemically Induced DNA Repair in Hepatocytes in Vivo and in Vitro As an Indicator of Carcinogenic Potential | 241 |
Chapter 11 Genotoxicity Studies with Human Hepatocytes | 265 |
| 281 | |
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7-hydroxycoumarin acetaminophen acid Acosta activity adriamycin adult rat hepatocytes animal assay Biochem Biol Biophys Bucher cadmium calcium Cancer Res carcinogens cellular Chem chemical concentration conjugation cultured hepatocytes cytochrome P-450 cytotoxicity cytotoxins decrease DNA repair DNA synthesis drug effects enzyme epidermal growth factor fraction function genotoxic glucagon glucose glucuronidation glucuronosyltransferase glutathione hepatic hepatocyte cultures hepatocyte DNA hepatotoxicity hormones human hepatocytes incubated indicators induced inhibition insulin interactions intracellular isolated hepatocytes isolated rat hepatocytes Kupffer cells levels lipid peroxidation liver liver cells liver lobule measured Mechanism medium metabolism metabolites Michalopoulos microsomal mitochondrial monolayer cultures mouse normal parenchymal cells partial hepatectomy perfused perfused livers pericentral pericentral areas pericentral regions periportal and pericentral Pharmacol postnatal primary cultures protein rat liver rates of glucuronidation receptors sensitive serum specific Stacey and Klaassen stimulation studies substrate suspensions tion tissue toxicity Toxicol trypan blue UDPGA uptake urea viability vitro vivo Williams xenobiotics