SN/T 1071-2014 Translated English of Chinese Standard. (SNT 1071-2014, SN/T1071-2014, SNT1071-2014): Method for the determination of anaerobic sulfite-reducing clostridia in food for exporthttps://www.chinesestandard.net, 10. aug. 2018 - 11 sider This standard specifies the detection method of anaerobic sulfite-reducing clostridia in food for export. This standard applies to the detection of anaerobic sulfite-reducing clostridia in food for export. |
Indhold
| 3 | |
Scope | 4 |
Media and reagents | 5 |
Testing steps and calculations | 6 |
Confirmation test | 7 |
Appendix A Normative Culture medium and reagents | 8 |
Appendix B Normative Most proximate number MPN of 1 g mL anaerobic sulfitereducing clostridia | 11 |
Almindelige termer og sætninger
1:10 sample homogenate 10-fold incremental dilution 5.0 g Sodium ADJUST the pH anaerobic culture anaerobic sulfite anaerobic sulfite-reducing clostridia Anhydrous potassium appropriate three consecutive Aseptically WEIGH 25 AUTOCLAVE barrier layer black colonies blank dilution Buffered glycerol-sodium chloride catalase CFU/g mL chloride tryptone dilute clostridia in food constant temperature water containing 225 mL corrosive samples culture medium determination of anaerobic diluted sample solution dishes for blank Distilled water food for export g Anhydrous potassium g Distilled g Yeast extract glycerol-sodium chloride solution Gram-positive HEAT to dissolve homogenized cup Ingredients Tryptone meat residue mL of blank mL of sodium MODIFY the numerical MPN/g mL Non-frozen and corrosive PIPETTE 1 mL plate counting method Preparation method MIX proximate number MPN respectively PIPETTE SN/T sodium chloride tryptone solution for sample spores sterile agar sterile homogenized sulfite agar temperature water bath test tubes three consecutive dilutions tryptone dilute solution typical colonies WEIGH 25 g